ABSTRACT
Objective To study the effect of survivin on temozolomide (TMZ) resistance of glioma cells and possible mechanism.Methods Survivin RNA and survivin shRNA plasmids were transfected into U87 cells,respectively.The cell growth curve drawn by MTT was used to test the changes in the proliferation of glioma cells.Colony forming assay was used to calculate cell surviving fraction.The Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) staining were applied to detect the apoptosis rate by flow cytometer (FCM).Western blotting was used to detect the expression of activated caspase-3.Results The cell proliferation and cell surviving fraction were inhibited by TMZ,and it was more significantly inhibited in TMZ plus down-regulation of survivin.Compared with parental cells,U87/sur si cells,treated by TMZ,showed a decrease in proliferation and colony forming efficiency and an increase in apoptosis rate,whereas U87/sur cells,treated by TMZ,showed an increase in proliferation and colony forming efficiency and a decrease in apoptosis rate.Western blotting showed that compared with TMZ alone,the expression of activated caspase-3 protein was promoted in TMZ plus down-regulation of survivin.Conclusion Down-regulation of survivin could enhance the sensitivity of glioma cells to TMZ,which may be related to survivin promoting cell apoptosis.
ABSTRACT
Objective To investigate the injury characteristics of Beagle dogs after warship multi cabin explosion.Methods Forty-eight adult male Beagle dogs were placed in the simulated blast-cabin and adjacent cabin (24 each),and ammunitions respectively containing 0.75kg TNT and 3.50kg TNT were then detonated in the blast-cabin.The survival situation,fluctuation of vital signs,morphological changes of organs and the incidence of various types of injury of the dogs were observed immediately after the explosion to 24h after injury,and the neurological functions score was performed.Results Twenty dogs died immediately after the explosion,and another 9 dogs died 24h after the explosion.The total mortality was 60.42％(29/48),and the mortalities in blastcabin and adjacent cabin were 79.17％(19/24) and 41.67％(10/24),respectively.The dog's skull was penetrated by bomb fragments,and congestion and bleeding were observed in brain tissue,lung,heart,stomach,bowels,liver and kidneys.Extremities fracture,soft tissue contusions,perforation and rupture were also checked out after explosion.The fatality rate of bomb fragment injury,blast injury and combined bomb fragment-blast injury was 27.59％(8/29),17.24％(5/29) and 55.17％(16/29),respectively.Conclusions The combined bomb fragment-blast injury show high incidence and make high fatality rate and serious injury.Combatants should effectually shield themselves with occluded objects as far as possible to avoid damage and reduce fatality.The key of early treatment is to treat the multiple injuries promptly.Intravenous fluid therapy should be practiced after hemodynamic monitoring.